NT-proBNP ELISA
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货号: |
SK-1204 |
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方法学: |
Sandwich ELISA, HRP/TMB, 12x8-well strips |
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样本类型: |
Serum and EDTA plasma |
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标准品范围: |
0-640 pmol/l (5 serum based standards) |
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标准品浓度: |
0/10/40/160/640 pmol/l |
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质控品: |
1 serum based control |
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样本量: |
50 μl / well |
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孵育时间: |
3 h / 30 min |
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单位换算: |
1 pg/ml = 0.118 pmol/l (MW: 8.475 kDa) |
灵敏度:
LOD: 3 pmol/l (0 pmol/l + 3 SD)
精密度：
Intra-assay (n=3) ≤ 3%, Inter-assay (n=8) ≤ 7%
回收率：
The mean recovery of recombinant NT-proBNP in serum samples (n=4) is 104%, in EDTA plasma samples (n=4) is 94%.
线性： Dilution linearity for serum samples (n=3) is between 79-86% for endogenous NT-proBNP (1-76) and 112-119% for recombinant NT-proBNP (1-76).
Dilution linearity for EDTA plasma samples (n=2) is between 83-84% for endogenous NT-proBNP (1-76) and 83-86% for recombinant NT-proBNP (1-76).
交叉反应:
The assay works with human samples only.
参考范围（健康人群）：
Median (serum, n=70): 5.8 pmol/l.
Median (EDTA plasma, n=28): 5.6 pmol/l.
推荐实验室建立自己的参考值。
仅供科研使用。
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