S100A8/S100A9 ELISA可用于检测小鼠，大鼠粪便，血清，尿液，组织提取液，细胞培养上清液中的钙卫蛋白MRP8/14 (S100A8/S100A9)。仅供科研使用。
样本制备
粪便样本：每个样本必须在提取缓冲液中以1:50的比例提取（例如100mg的粪便+ 5 ml提取缓冲液），然后在3000g离心10分钟。为便于进行分析，每孔移取100μl上清液。
EDTA-血浆/血清样本： 在测定之前，样本应用洗涤缓冲液按1：100稀释。为便于进行分析，每孔移取100μl稀释液。
尿液样本： 在测定前，样本应至少用洗涤缓冲液以1：3的比例稀释。为便于进行分析，每孔移取100μl稀释液。
细胞培养上清液样本： 在测定之前，样本应至少用洗涤缓冲液以1：2的比例稀释。为便于进行分析，每孔移取100μl稀释液。
参考文献
1. Hildebrand, F. et al., 2013. Inflammation-associated enterotypes, host genotype, cage and inter-individual effects drive gut microbiota variation in common laboratory mice. Genome biology, 14(1), p.R4.
2. Nelson, D. a et al., 2012. An expanded myeloid derived suppressor cell population does not play a role in gammaherpesvirus-exacerbated breast cancer metastases. Infectious agents and cancer, 7(1), p.22. Manual S100A8/S100A9 (MRP8/14) 24
3. Prinzen, C. et al., 2009. Differential gene expression in ADAM10 and mutant ADAM10 transgenic mice. BMC genomics, 10, p.66.
4. Soro-Paavonen, A. et al., 2008. Receptor for advanced glycation end products (RAGE) deficiency attenuates the development of atherosclerosis in diabetes. Diabetes, 57(9), pp.2461–2469.
5. Volz, H.C. et al., 2012. S100A8/A9 aggravates post-ischemic heart failure through activation of RAGE-dependent NF-κB signaling. Basic research in cardiology, 107(2), p.250.
6. Wiechert, L. et al., 2012. Hepatocyte-specific S100a8 and S100a9 transgene expression in mice causes Cxcl1 induction and systemic neutrophil enrichment. Cell communication and signaling : CCS, 10(1), p.40.
7. Yamamoto, Y. et al., 2011. Septic shock is associated with receptor for advanced glycation end products ligation of LPS. Journal of immunology (Baltimore, Md. : 1950), 186(5), pp.3248–57.
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