This 2nd generation Human FGF-23 (Intact) ELISA Kit is a two-site enzyme-linked immunosorbent assay (ELISA) for the measurement of FGF-23 in plasma or cell culture media. A murine monoclonal antibody and an affinity purified goat polyclonal antibody have been selected to detect epitopes within the amino terminal and carboxyl-terminal portions of FGF-23. The monoclonal antibody is biotinylated for capture and the other antibody is conjugated with the enzyme horseradish peroxidase (HRP) for detection.