This 2nd generation Human FGF-23 (C-Term) ELISA Kit is a two-site enzyme-linked immunosorbent assay (ELISA) for the measurement of FGF-23 in plasma or cell culture media. Two affinity purified goat polyclonal antibodies have been selected to detect epitopes within the carboxyl-terminal (C-Term) portion of FGF-23. One antibody is biotinylated for capture and the other antibody is conjugated with the enzyme horseradish peroxidase (HRP) for detection. These antibodies bind to both the intact molecule and large carboxyl terminal fragments of human FGF-23.