The Rat Intact PTH ELISA Kit is a two-site enzyme-linked immunosorbent assay (ELISA) for the measurement of PTH in rat serum, plasma or cell culture media. Two different goat polyclonal antibodies to rat intact PTH have been purified by affinity chromatography. The antibody which recognizes epitopes within the midregion/C-terminal portion (39-84) of the peptide is biotinylated for capture. The other antibody which recognizes epitopes within the N-terminal region (1-34) is conjugated with the enzyme horseradish peroxidase (HRP) for detection.