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25(OH) VITAMIN D
ANTIBODY, MONOCLONAL
 
Catalog no.: A 1025.1 / A 1025.2
 
Immunogen: 25(OH) D3 3-Hemisuccinat-BSA
 
Synonyms: Calcidiol, 25-hydroxycholecalciferol 
 
Host: Mouse Balb/c
 
Clone no.: ID1, 25B
 
Isotype: IgG1
 
Matrix: Cell culture supermnatant, Protein G purified, PBS pH 7.4, 2% BSA
 
Specificity: Cross reactivity (determined with ELISA K 2109):
25(OH) D3 (Calcidiol):                                                        100%
25(OH) D2 (Ergocalcidiol):                                                   68%
24,25(OH)2 D3 (24,25-Dihydroxycholecalciferol):           ≥ 100%
D3 (Cholecalciferol):                                                         < 0.1%
D2 (Ergocalciferol):                                                              0.3%
There was no cross reactivity obtained with BSA.
 
Contents: 10 μg / 100 μg (lyophilized)
Resuspend in 10 μl / 100 μl aqua bidest.
 
Known applications: ELISA (1:15 000-50 000)
This antibody has not been tested for use in all applications. This does not necessarily exclude its use in non-tested procedures. The stated dilutions are recommendations only. End users should determine optimal dilutions in their system using appropriate negative/positive controls.
 
Store at: 2-8 °C (lyophilized);- 20 °C (liquid)
Repeated thawing and freezing must be avoided
 
For research use only
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