ANTIBODY, POLYCLONAL
Catalog no.: AS1016.3 / AS1016.4
Immunogen: Recombinant bovine Vascular Endothelial Growth Factor-164
Synonyms: Vascular endothelial growth factor A (VEGF-A), Vascular permeability factor (VPF)
Swiss-Prot No: P15691
Gene Information: Gene Name: VEGF, VEGFA
Gene ID: 281572
Host: Rabbit
Matrix: IgG, Protein G purified from serum; 50 mM TRIS, pH 7.4
Specificity: Bovine, porcine, and domestic ruminants VEGF-164, and all human VEGF isoforms (121, 165, 189, and 206)
No cross reaction was obtained with PDGF-AA, PDGF-BB, PDGF-AB, FGF-1, FGF-2, and TGFα.
Contents: 10 μg / 100 μg (lyophilized)
Resuspend in 10 μl / 100 μl aqua bidest.
Known applications: RIA1, 2, 3, 4, immunohistochemistry (paraffin sections; cryosections)1, 2, 3, Western Blot2, 5
This antibody has not been tested for use in all applications. This does not necessarily exclude its use in non-tested procedures. The stated dilutions are recommendations only. End users should determine optimal dilutions in their system using appropriate negative/positive controls.
Store at: 2-8 °C (lyophilized); - 20 °C (dissolved)
Repeated thawing and freezing must be avoided
References:
1. Berisha B, Schams D, Kosmann M, Amselgruber W, Einspanier R (2000). Expression and localization of vascular endothelial growth factor and basic fibroblast growth factor during the final growth of bovine ovarian follicles. J Endocrinol 167(3): 371-382.
2. Berisha B, Schams D, Kosmann M, Amselgruber W, Einspanier R (2000). Expression and tissue concentration of vascular endothelial growth factor, its receptors, and localization in the bovine corpus luteum during estrous cycle and pregnancy. Biol Reprod 63(4): 1106-1114.
3. Einspanier R, Schonfelder M, Muller K, Stojkovic M, Kosmann M, Wolf E, Schams D (2002). Expression of the vascular endothelial growth factor and its receptors and effects of VEGF during in vitro maturation of bovine cumulus-oocyte complexes (COC). Mol Reprod Dev 62(1): 29-36.
4. Kaczmarek MM, Blitek A, Schams D, Ziecik AJ (2010). Effect of Luteinizing Hormone and Tumour Necrosis Factor-Alpha on VEGF Secretion by Cultured Porcine Endometrial Stromal Cells. Reproduction in Domestic Animals 45(3): 481-486.
5. Klipper E, Levit A, Mastich Y, Berisha B, Schams D, Meidan R (2010). Induction of Endothelin-2 Expression by Luteinizing Hormone and Hypoxia: Possible Role in Bovine Corpus Luteum Formation. Endocrinology 151(4): 1914-1922.
For research use only
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