ANTIBODY, POLYCLONAL
Catalog no.: A 9520.1 / A 9520.2
Immunogen: Synthetic human Thymosin β4 (aa 1-14) KLH conjugated (AcSDKPDMAEIEKFDK)
Synonyms: T beta-4, Fx
Swiss-Prot No: P62328
Gene Information: Gene name: TMSB4X, TB4X, THYB4, TMSB4
GenelD: 7114
Host: Rabbit
Matrix: Serum
Specificity: Human Thymosin β4 (aa 1-14), human and mouse Thymosin β4.
There was no cross reactivity obtained with human Thymosin a1 and human Thymulin, Tb4 (aa 1-4), Tb9, Tb9 (aa 1-14), AcADKP, ADKP, AcGDKP, AcTDKP, AcSPKD, AcVDKP, BSA.
Contents: 20 μl / 100 μl (lyophilized)
Resuspend in 20 μl / 100 μl aqua bidest.
Known applications: ELISA (1:4000)1, 2, Western Blot (1:5000)8, dot blot (1:500), immunohistochemistry (paraffin sections, 1:200; cryosections, 1:100; vibratome sections, 1:8000; whole-mount, 1:100)3, 4, 5, 6, 7, 8
This antibody has not been tested for use in all applications. This does not necessarily exclude its use for non-tested procedures. The stated dilutions are recommendations only. We suggest that the applicant titrates the antibody in his/her system using appropriate negative/ positive controls.
Store at: 2-8 °C (lyophilized); - 20 °C (dissolved)
Repeated thawing and freezing must be avoided
References:
1. Livaniou E, Mihelic M, Evangelatos GP, Haritos A, Voelter W (1992). A thymosin beta 4 ELISA using an antibody against the N terminal fragment thymosin beta 4 [1-14]. J Immunol Methods 148(1-2): 9-14.
2. Becker S, Armbruster FP, Muller B, Echner H, Kapurnotu A, Livaniou E, Mihelic M, Stoeva S, Voelter W (1994). Theoretical and experimental epitope mapping of thymosin beta 4. J Immunol Methods 177(1-2): 131-137.
3. Mihelic M, Livaniou E, Horger S, Galic M, Giebel W, Lenfant M, Voelter W. Antibodies against Thymosin beta4-Their Specificity and use in Immunohistochemical Studies. Recent Advances in Cellular and Molecular Biology Abstract Book from the 1. World Congress of C.M.B, Paris, September 1-7, 1991 1, 43-49. 1992.
4. Larsson LI, Holck S (2007). Localization of thymosin beta-4 in tumors. Ann N Y Acad Sci 1112: 317-325.
5. Paulussen M, Landuyt B, Schoofs L, Luyten W, Arckens L (2009). Thymosin beta 4 mRNA and peptide expression in phagocytic cells of different mouse tissues. Peptides 30(10): 1822-1832.
6. Smart N, Dube KN, Riley PR (2010). ldentification of Thymosin beta4 as an effector of Hand1-mediated vascular development. Nat Commun 1(4): 1-10.
7. Gruner BM, Hahne H, Mazur PK, Trajkovic-Arsic M, Maier S, Esposito I, Kalideris E, Michalski CW, Kleeff J, Rauser S, Schmid RM, Kuster B, Walch A, Siveke JT (2012). MALDI imaging mass spectrometry for in situ proteomic analysis of preneoplastic lesions in pancreatic cancer. PLoS One 7(6): e39424.
8. Kim J, Hyun J, Wang S, Lee C, Lee J-W, Moon E-Y, Cha H, Diehl AM & Jung Y (2017). Thymosin beta-4 regulates activation of hepatic stellate cells via hedgehog signaling. Sci. Rep.7: 3815.
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