Known applications: ELISA (1:10 000), immunohistochemistry (vibratome sections, 1:500-1000; cryosections, 1:300)2, 3, 4
Do not fix specimen in methanol for immunohistochemistry
This antibody has not been tested for use in all applications. This does not necessarily exclude its use for non-tested procedures. The stated dilutions are recommendations only. We suggest that the applicant titrates the antibody in his/her system using appropriate negative/positive controls.
Store at: 2-8 °C (lyophilized); - 20 °C (dissolved)
Repeated thawing and freezing must be avoided
References:
1. Anavi-Goffer S, Coutts AA (2003). Cellular distribution of vanilloid VR1 receptor immunoreactivity in the guinea-pig myenteric plexus. Eur J Pharmacol 458(1-2):61-71.
2. Patte-Mensah C, Kibaly C, Mensah-Nyagan AG (2005). Substance P inhibits progesterone conversion to neuroactive metabolites in spinal sensory circuit: a potential component of nociception. Proc Natl Acad Sci USA 102(25): 9044-9049.
3. Papp I, Szucs P, Hollo K, Erdelyi F, Szabo G, Antal M (2006). Hyperpolarization-activated and cyclic nucleotide-gated cation channel subunit 2 ion channels modulate synaptic transmission from nociceptive primary afferents containing substance P to secondary sensory neurons in laminae l-llo of the rodent spinal dorsal horn. Eur J Neurosci 24(5): 1341-1352.
4. Hamamoto T, Takumida M, Hirakawa K, Takeno S, Tatsukawa T (2008). Localization of transient receptor potential channel vanilloid subfamilies in the mouse larynx. Acta Otolaryngol 128(6): 685-693.
For research use only
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