ANTIBODY, POLYCLONAL
Catalog no.: AC1008.1 / AC1008.2
Immunogen: Purified filaments of spicules from Suberites domuncula
Swiss-Prot No: Q2MEV3 and Q70TB1
Gene Information: Gene Name: silicaa-g and silicb
Host: Rabbit
Matrix: Serum
Specificity: Silicatein-α and silicatein-β, tested against following species: Suberites domuncula, Lubomirskia baikalensis, Tethya aurantium, Geodia cydonium
Contents: 20 μl / 100 μl (lyophilized)
Resuspend in 20 μl / 100 μl aqua bidest.
Known applications: ELISA (1:6000), Western Blot (1:1500)1, immunohistochemistry (resin sections, 1:800)1, immunoprecipitation1
This antibody has not been tested for use in all applications. This does not necessarily exclude its use for non-tested procedures. The stated dilutions are recommendations only. We suggest that the applicant titrates the antibody in his/her system using appropriate negative/positive controls.
Store at: 2-8 °C (lyophilized); - 20 °C (dissolved)
Repeated thawing and freezing must be avoided
References:
1. Schroder HC, Boreiko A, Korzhev M, Tahir MN, Tremel W, Eckert C, Ushijima H, Muller IM, Muller WEG (2006). Co-expression and Functional Interaction of silicatein with galectin, matrix-guided formation of siliceous spicules in the marine demosponge Suberites domuncula. J. Biol. Chem. 281: 12001-12009.
2. Muller WEG, Belikov SI, Tremel W, Perry C, Gieskes WWC, Boreiko A, Schroder HC (2006). Siliceous spicules in marine demosponges (example Suberites domuncula. Micron 37: 107-120.
3. Muller WEG, Rothenberger M, Boreiko A, Tremel W, Reiber A, Schroder HC (2005). Formation of siliceous spicules in the marine demosponge Suberites domuncula. Cell Tissue Res.321: 285-297.
For research use only