ANTIBODY, POLYCLONAL
Catalog no.: A 9211.3 / A 9211.4
Immunogen: Purified human Relaxin-2
Swiss-Prot No: P04090
Gene Information: Gene Name: RLN2
GenelD: 6019
Host: Rabbit
Matrix: IgG, Protein G purified from serum; 50 mM TRIS pH 7.5
Specificity: Human Relaxin-1 and Relaxin-2, pro-Relaxin-1 and pro-Relaxin-2.
No cross reactivity was obtained with the following proteins:
Insulin, Zn-Insulin, IGF-1, IGF-2, Spermolaxin, Inhibin α-subunit, Inhibin, Seminal-Plasma-Inhibin-like peptide, CG, LH, FSH, Prolactin and Ubiquitin.
Contents: 10 μg / 100 μg (lyophilized)
Resuspend in 10 μl / 100 μl aqua bidest.
Known applications: RIA (1 μg/ml)1, 3, ELISA (1 μg/ml) 2, 4, 5, immunohistochemistry (paraffin sections; cryosections, 5 μg/ml)4, 6, 8, 9, Western Blot4, 7, immunoprecipitation7
This antibody has not been tested for use in all applications. This does not necessarily exclude its use in non-tested procedures. The stated dilutions are recommendations only. End users should determine optimal dilutions in their system using appropriate negative/positive controls.
Store at: 2-8 °C (lyophilized); - 20 °C (dissolved)
Repeated thawing and freezing must be avoided
References:
1. Meisenbach M, Armbruster FP, Becker S, Gron HJ, Grubler T, Lippert TH, Paulus G, and Volter W (1995). Development and characterization of antibodies specific for human relaxin 2 (hRLX-2). In PTP. Kaumaya, RS. Hodges (Eds.) Peptides. Chemistry, Structure and Biology (Proceedings of the 14th American Peptide Symposium) Mayflower Scientific Ltd. England.
2. Armbruster FP, Maier I, Gron HJ et al. (2000). A highly sensitive homologous human relaxin ELISA. In GW.Tregear et al (eds.) Relaxin 2000: Proceedings of the third International Conference on Relaxin & Related Peptides, p 273-274.
3. Armbruster FP, Gron HJ, Maier I, Becker S, Bailer SM, Lippert TH, Seeger H, Kloppinger M, Tampe J, Stoeva S, Voelter W (2001). A sensitive homologous radioimmunoassay for human relaxin-2 (h-RLX-2) based on antibodies characterized by epitope mapping studies. Eur J Med Res 6(1): 1-9.
4. Dschietzig T, Richter C, Bartsch C, Laule M, Armbruster FP, Baumann G, Stangl K. (2001). The pregnancy hormone relaxin is a player in human heart failure. FASEB Journal 15: 2187-95.
5. Vogel I, Glavind-Kristensen M, Thorsen P, Armbruster FP, Uldbjerg N (2002). S-relaxin as a predictor of preterm delivery in women with symptoms of preterm labour. BJOG 109(9): 977-982.
6. Galey S, Konieczko EM, Arnold CA, Cooney TE (2003). Immunohistological detection of relaxin binding to anterior cruciate ligaments. Orthopedics 26(12): 1201-1204.
7. Dschietzig T, Bartsch C, Greinwald M, Baumann G, Stangl K (2005). The pregnancy hormone relaxin binds to and activates the human glucocorticoid receptor. Ann N Y Acad Sci 1041: 256-271.
8. Shirota K, Tateishi K, Koji T, Hishikawa Y, Hachisuga T, Kuroki M, Kawarabayashi T (2005). Early Human Preantral Follicles Have Relaxin and Relaxin Receptor (LGR7), and Relaxin Promotes Their Development. J Clin Endocrinol Metab 90(1): 516-521.
9. Lubahn J, Ivance D, Konieczko E, Cooney T (2006) Immunohistochemical detection of relaxin binding to the volar oblique ligament. J Hand Surg Am 31(1): 80-84.
For research use only
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