Contents: 20 μl / 100 μl (lyophilized)
Resuspend in 20 μl / 100 μl aqua bidest.
Known applications: ELISA (1:5000)1, Western Blot (1:100; 5 μg/ml purified lgG)1, immunohistochemistry (paraffin sections, 5-10 μg/ml; cryosections)3
This antibody has not been tested for use in all applications. This does not necessarily exclude its use for non-tested procedures. The stated dilutions are recommendations only. We suggest that the applicant titrates the antibody in his/her system using appropriate negative/positive controls.
Store at: 2-8 °C (lyophilized);- 20 °C (dissolved)
Repeated thawing and freezing must be avoided
References:
1. Reinartz J, Schaefer B, Bechtel MJ, Kramer MD (1996). Plasminogen activator inhibitor type-2 (PAI-2) in human keratinocytes regulates pericellular urokinase type plasminogen activator. Exp Cell Res 223(1): 91-101.
2. Ferrier CM, van Geloof WL, de Witte HH, Kramer MD, Ruiter DJ, van Muijen GN (1998). Epitopes of components of the plasminogen activation system are re-exposed in formalin-fixed paraffin sections by different retrieval techniques. J Histochem Cytochem 46(4): 469-476.
3. Bechtel MJ, Wysocki NS, Heidtmann A, Stark HJ, Fusenig N, Kramer MD, Schaefer BM (1998). Plasminogen activator inhibitor type 2 is expressed in keratinocytes during re-epithelialization of epidermal defects. Br J Dermatol 138(1): 22-28.
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