ANTIBODY, MONOCLONAL
Catalog no.: AK1104.1 / AK1104.2
Immunogen: Synthetic human PTH peptide (aa 1-38) polyLysine conjugated (SVSEIQLMHNLGKHLNSMERVEWLRKKLQDVHNFVALG)
Synonyms: Parathyrin, Parathormone
Swiss-Prot No: P01270
Gene Information: Gene name: PTH
GeneID: 5741
Host: Mouse Balb/c
Clone no.: A1/70
Isotype: IgG1
Matrix: Cell culture supernatant, Prot. G purified; PBS pH 7.4
Specificity: Human PTH peptides (aa 15-25; 1-34; 1-38; 1-84; 7-84)
Affinity: 1.47 x 108 l/mol (determined by RIA)
There was no cross reactivity obtained with synthetic human PTH (aa 1-3; 1-10; 4-16; 28-48; 39-84; 44-68; 53-84), nor with PTHrP (aa 1-86), Calcitonin, Gastrin, @2 microglobulin, Thymulin, Thyroglobulin, Streptavidin, or Glutathione S-transferase.
Contents: 10 μg / 100 μg (lyophilized)
Resuspend in 10 μl / 100 μl aqua bidest.
Known applications: RIA (20 ng/ml), ELISA (1 μg/ml)1, ILMA (20 μg/ml)3, immunohistochemistry
(paraffin sections, cryosections, 2 μg/ml)
This antibody has not been tested for use in all applications. This does not necessarily exclude its use in non-tested procedures. The stated dilutions are recommendations only. End users should determine optimal dilutions in their system using appropriate negative/positive controls.
Store at: 2-8 °C (lyophilized); - 20 °C (dissolved)
Repeated thawing and freezing must be avoided
References:
1. Tampe J, Broszio P, Manneck HE, Missbichler A, Blind E, Muller KB, Schmidt-Gayk H, Armbruster FP
(1992). Characterization of antibodies against human N-terminal parathyroid hormone by epitope mapping. J Immunoassay 13(1): 1-13.
2. Klaus G, von Eichel B, May T, Hugel U, Mayer H, Ritz E, Mehls O (1994). Synergistic effects of parathyroid hormone and 1,25-dihydroxyvitamin D3 on proliferation and vitamin D receptor expression of rat growth cartilage cells. Endocrinology 135(4): 1307-1315.
3. Gao P, Schmidt-Gayk H, Dittrich K, Nolting B, Maier A, Roth HJ, Seemann O, Reichel H, Ritz E, Schilling T (1996). Immunochemiluminometric assay with two monoclonal antibodies against the N-terminal sequence of human parathyroid hormone. Clin Chim Acta 245(1): 39-59.
For research use only
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