Known applications: RIA (1:80 000)1, 2, 3, 4, 5, Western Blot, immunohistochemistry (paraffin sections, 1:500)5
This antibody has not been tested for use in all applications. This does not necessarily exclude its use for non-tested procedures. The stated dilutions are recommendations only. We suggest that the applicant titrates the antibody in his/her system using appropriate negative/positive controls.
Store at: 2-8 °C (lyophilized); - 20 °C (dissolved)
Repeated thawing and freezing must be avoided
References:
1. Schams D, Schmidt-Polex B, Kruse V (1979). Oxytocin determination by radioimmunoassay in cattle. I. Method and preliminary physiological data. Acta Endocrino/ 92(2):258-270.
2. Schams D, Lahlou-Kassi A, Glatzel P (1982). Oxytocin concentrations in peripheral blood during the oestrous cycle and after ovariectomy in two breeds of sheep with low and high fecundity. J Endocrinol 92(1):9-13.
3. Schams D (1983). Oxytocin determination by radioimmunoassay. III. Improvement to subpicogram sensitivity and application to blood levels in cyclic cattle. Acta Endocrinol 103(2):180-183.
4. Walters DL, Schams D, Schallenberger E (1984). Pulsatile secretion of gonadotrophins, ovarian steroids and ovarian oxytocin during the luteal phase of the oestrous cycle in the cow. J Reprod Fertil 71 (2):479-491.
5. Kruip TA, Vullings HG, Schams D, Jonis J, Klarenbeek A (1985). Immunocytochemical demonstration of oxytocin in bovine ovarian tissues. Acta Endocrinol 109(4):537-542.
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