Contents: 20 μl / 100 μl (lyophilized)
Resuspend in 20 μl / 100 μl aqua bidest.
Known applications: ELISA (1:5,000)1, Western Blot (1:2000)2, 3, immunohistochemistry (paraffin sections, 1:1000)
This antibody has not been tested for use in all applications. This does not necessarily exclude its use in non-tested procedures. The stated dilutions are recommendations only. End users should determine optimal dilutions in their system using appropriate negative/positive controls.
Store at: 2-8 °C (lyophilized); - 20 °C (dissolved)
Repeated thawing and freezing must be avoided
References:
1. Withold W, Friedrich W, Degenhardt S (1997). Serum bone alkaline phosphatase is superior to plasma levels of bone matrix proteins for assessment of bone metabolism in patients receiving renal transplants. Clinica Chimica Acta 261(2): 105-115.
2. Kaufmann B, Muller S, Hanisch FG, Hartmann U, Paulsson M, Maurer P, Zaucke F (2004). Structural variability of BM-40/SPARC/osteonectin glycosylation: implications for collagen affinity. Glycobiology 14(7): 609-619.
3. Adwan H, Bauerle TJ, Berger MR (2004). Downregulation of osteopontin and bone sialoprotein II is related to reduced colony formation and metastasis formation of MDA-MB-231 human breast cancer cells. Cancer Gene Ther 11(2): 109-120.
For research use only
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