yl23411永利(中国)有限公司-搜狗百科

Known applications: ELISA (1:5000-16 000)1, 2, RIA1, 2, 3, Western Blot (1:400-1000)3, 4, 5, immunohistochemistry (paraffin sections; cryosections, 4-10 μg/ml antigen affinity-purified antibody)1, 2, 3, 4

This antibody has not been tested for use in all applications. This does not necessarily exclude its use in non-tested procedures. The stated dilutions are recommendations only. End users should determine optimal dilutions in their system using appropriate negative/positive controls.

Store at: 2-8 °C (lyophilized); - 20 °C (dissolved)

Repeated thawing and freezing must be avoided

Reference List:

1. Kohfeldt E, Sasaki T, Gohring W, Timpl R (1998). Nidogen-2: a new basement membrane protein with diverse binding properties. J Mol Biol 282:99-109.

2. Salmivirta K, Talts JF, OIsson M, Sasaki T, Timpl R, Ekblom P (2002). Binding of mouse nidogen-2 to basement membrane components and cells and its expression in embryonic and adult tissues suggest complementary functions of the two nidogens. Exp Cell Res 279:188-201.

3. Schymeinsky J, Nedbal S, Miosge N, Poschl E, Rao C, Beier DR, Skarnes WC, Timpl R, Bader BL (2002). Gene structure and functional analysis of the mouse nidogen-2 gene: nidogen-2 is not essential for basement membrane formation in mice. Mol Cell Biol 22(19): 6820-6830.

4. Kunze A, Abari E, Semkova I, Paulsson M, Hartmann U (2010). Deposition of nidogens and other basement membrane proteins in the young and aging mouse retina. Ophthalmic Res 43(2): 108-112.

5. Lutter S, Xie S, Tatin F, Makinen T (2012). Smooth muscle-endothelial cell communication activates Reelin signaling and regulates lymphatic vessel formation. J Cell Biol 197(6): 837-849.

For research use only