Specificity: MDA-modified proteins, especially dihydropyridine (DHP)-lysine type derivative
Contents: 30 μg (frozen; 100 μg/ml)
Known applications: Western Blot (2 μg/ml)1, immunohistochemistry (paraffin sections, 0.5-1.0 μg/ml; cryosections)1, immunofluorescence2
This antibody has not been tested for use in all applications. This does not necessarily exclude its use in non-tested procedures. The stated dilutions are recommendations only. End users should determine optimal dilutions in their system using appropriate negative/positive controls.
Store at: - 20 °C
Repeated thawing and freezing must be avoided
References:
1. Yamada S, Kumazawa S, Ishii T, Nakayama T, Itakura K, Shibata N, Kobayashi M, Sakai K, Osawa T, Uchida K (2001). Immunochemical detection of a lipofuscin-like fluorophore derived from malondialdehyde and lysine. J Lipid Res 42(8): 1187-1196.
2. Muratori, Monica, Lara Tamburrino, Sara Marchiani, Marta Cambi, Biagio Olivito, Chiara Azzari, Gianni Forti, and Elisabetta Baldi. (2015). Investigation on the Origin of Sperm DNA Fragmentation: Role of Apoptosis, Immaturity and Oxidative Stress. Molecular Medicine 21 (1): 109–22.