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HUMAN LAMININ 5
ANTIBODY, MONOCLONAL
 
Catalog no.: AP1008.1 / AP1008.2
 
Immunogen: Human Laminin 5 beta 3 chain
 
Synonyms: Laminin-5 subunit beta, Epiligrin subunit beta, Kalinin subunit beta, Nicein subunit beta, Laminin B1k chain, Kalinin B1 chain
 
Swiss-Prot No: Q13751
 
Gene Information: Gene Name: LAMB3, LAMB1 
Gene ID:3914
 
Host: Mouse Balb/c
 
Clone no.: 6F12
 
Isotype: IgG
 
Matrix: Cell culture supernatant, Protein A purified, PBS pH 7.4
 
Specificity: Human Laminin 5, rat Laminin 5
 
Contents: 10 μg / 100 μg (lyophilized)
Resuspend in aqua bidest.
 
Known applications: Western Blot1, ELISA (secondary antibody in sandwich ELISA)3, 5 immunohistochemistry (cryosections)2, 4, 6
This antibody has not been tested for use in all applications. This does not necessarily exclude its use in non-tested procedures. The stated dilutions are recommendations only. End users should determine optimal dilutions in their system using appropriate negative/positive controls.
 
Store at: 2-8 °C (lyophilized); - 20 °C (dissolved)
Repeated thawing and freezing must be avoided
 
References:
1. Marinkovich MP, Lunstrum GP, Burgeson RE (1992). The anchoring filament protein kalinin is synthesized and secreted as a high molecular weight precursor. J Biol Chem 267(25): 17900-17906.
2. Sasaki T, Amano S, Nishiyama T, Nakayama Y, Burgeson RE, Nakajima H (1998). Expression of laminin 5, and collagen IV and VII in bullous pemphigoid skin. Arch Dermatol Res 290(5): 283-285.
3. Amano S, Nishiyama T, Burgeson RE (1999). A specific and sensitive ELISA for laminin 5. J Immunol Methods 224(1-2): 161-169.
4. Libby RT, Champliaud M-F, Claudepierre T, Xu Y, Gibbons EP, Koch M, Burgeson RE, Hunter DD, Brunken WJ (2000). Laminin Expression in Adult and Developing Retinae: Evidence of Two Novel CNS Laminins. The Journal of Neuroscience 20(17): 6517-6528.
5. Amano S, Akutsu N, Ogura Y, Nishiyama T (2004.) Increase of laminin 5 synthesis in human keratinocytes by acute wound fluid, inflammatory cytokines and growth factors, and lysophospholipids. Br J Dermatol 157(5): 961-970.
6. Drumea-Mirancea M, Wessels JT, Miller CA, EssI M, Eble JA, Tolosa E, Koch M, Reinhardt DP, Sixt M, Sorokin L, Stierhof Y-D, Schwarz H, Klein G (2006). Characterization of a conduit system containing laminin-5 in the human thymus: a potential transport system for small molecules. Journal of Cell Science 119(7): 1396-1405.
 
For research use only

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