Known applications: Western Blot1, 2, 5, 7, immunoprecipitation3, immunohistochemistry (cryosections, methano-fixed, 1.5-3 μg/ml) 3, 4, 6, 7, 8, immunocytochemistry5, 7
This antibody has not been tested for use in all applications. This does not necessarily exclude its use in non-tested procedures. The stated dilutions are recommendations only. End users should determine optimal dilutions/concentrations their system using appropriate negative/positive controls.
Store at: 2-8 °C (lyophilized); - 20 °C (dissolved)
Repeated thawing and freezing must be avoided
References:
1. Marinkovich MP, Lunstrum GP, Burgeson RE (1992). The anchoring filament protein kalinin is synthesized and secreted as a high molecular weight precursor. J Biol Cherm 267(25): 17900-17906.
2. Zllikens D, Kawahara Y, Ishiko A, Shimizu H, Mayer J, Rank CV, Liu Z Giudice GJ, Tran HH, Marinkovich MP, Brocker EB, Hashimoto T (1996). A novel subepidermal blistering disease with autoantibodies to a 200-kDa antigen of the basement membrane zone. J Invest Dermatol 106(6): 133-1338.
3. Amano S, Scott IC, Takahara K, Koch M, Champliaud MF, Gerecke DR, Keene DR, Hudson DL, Nishiyama T, Lee S, Greenspan DS, Burgeson RE (2000). Bone morphogenetic protein 1 is an extracellular processing enzyme of the laminin 5 gamma 2 chain. J Biol Chem 275(30): 22728-22735.
4. Libby RT, Champliaud M-F, Claudepierre T, Xu Y, Gibbons EP, Koch M, Burgeson RE, Hunter DD, Brunken WJ (2000). Laminin Expression in Adult and Developing Retinae: Evidence of Two Novel CNS Laminins. The Journal of Neuroscience 20(17): 6517-6528.
5. Waterman EA, Sakai N, Nguyen NT, Horst BAJ, Veitch DP, Dey CN, Ortiz-Urda S, Khavari PA, Marinkovich MP (2007). A Laminin-Collagen Complex Drives Human Epidermal Carcinogenesis through Phosphoinositol-3-Kinase Activation. Cancer Research 67(9): 4264-4270.
6. Choi SK, Kim JH, Lee D, Lee JB, Kim HM, Tchah HW, Hahn TW, Joo M, Ha CI (2008). Different epithelial cleavage planes produced by various epikeratomes in epithelial laser in situ keratomileusis. Journalof Cataract & Refractive Surgery 34(12): 2079 2084.
7. Tran M, Rousselle P, Nokelainen P, Tallapragada S, Nguyen NT, Fincher EF, Marinkovich MP (2008). Targeting a Tumor-Specific Laminin Domain Critical for Human Carcinogenesis. Cancer Research 68(8): 2885- 2894.
8. Song J, Lokmic Z, Lammermann T, Rolf J, Wu C, Zhang X, Hallmann R, Hannocks MJ, Horn N, Ruegg MA, Sonnenberg A, Georges-Labouesse E, Winkler TH, Kearney JF, Cardell S, Sorokin L (2013). Extracellular matrix of secondary lymphoid organs impacts on B-cell fate and survival. Proc Natl Acad SciUSA 110(31): E2915-E2924.
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