ANTIBODY, MONOCLONAL
Catalog no.: AC1012.1 / AC1012.2
Immunogen: HSP70 from Geodia cydonium
Synonyms: Heat shock protein 70
Swiss-Prot No: Q24952
Gene Information: Gene Name: hsp70
Host: Mouse Balb/c
Clone no.: IVF5
Isotype: IgG1
Matrix: Cell culture supernatant, Protein G purified, 50mM TRIS pH 7.4
Specificity: HSP70
Contents: 10 μl / 100 μl (lyophilized)
Resuspend in 10 μl / 100 μl aqua bidest.
Known applications: ELISA (1:5000)3, Western Blot (1:500)2, 3, immunoprecipitation, immunohistochemistry (1:50)
This antibody has not been tested for use in all applications. This does not necessarily exclude its use for non-tested procedures. The stated dilutions are recommendations only. We suggest that the applicant titrates the antibody in his/her system using appropriate negative/ positive controls.
Store at: 2-8 °C (lyophilized); - 20 °C (dissolved)
Repeated thawing and freezing must be avoided
References:
1. Koziol C, Kruse M, Wagner-Hulsmann C, Pancer Z, Batel R and Muller WEG (1996). Cloning of the
heat-inducible stress protein (hsp70) from the marine sponge Geodia cydonium. Canad Technical Rep
Fisheries Aquatic Sci 2093: 104-109.
2. Schroder HC, Hassanein HMA, Lauenroth S, Koziol C, Mohamed TA-AA, Lacorn M, Steinhart H, Batel R and Muller WEG (1999). Induction of DNA strand breaks and expression of HSP70 and GRP78 homolog by cadmium in the marine sponge Suberites domuncula. Arch. Environ. Contam. Toxicol. 36: 47-55.
3. Wiens M, Koziol C, Hassanein HMA, Batel R, Sdhroder HC and Muller WEG (1998). Induction of gene
expression of the chaperones 14-3-3 and HSP70 by PCB118 (2,3',4,4,5-pentachlorobiphenyI) in the
marine sponge Geodia cydonium: novel biomarkers for polychlorinated biphenyls. Mar. Ecol. Prog. Ser.
165: 247-257.
For research use only