ANTIBODY, MONOCLONAL
Catalog no.: A6104.1 / A6104.2
Immunogen: Synthetic human GLP-1 (aa 7-36) amide KLH-conjugated
Swiss- Prot No: P01275
Gene Information: Gene Name: GCG
GenelD: 2641
Host: Mouse Balb/c
Clone no: ID/GLP-1/18
Isotype: lgG1
Matrix: Cell culture supernatant, Protein G purified, PBS pH 7.4
Specificity: GLP-1 (aa 7-36) amide; GLP-1 (aa 1-36) amide, acety| GLP-1 (aa 7-34) amide; human, rat, porcine, and chicken GLP-1
There was no σoss reactivity obtained with GLP-2, GRPP, PYY, VIP, PHI, GIP, glucagon, oxyntomodulin.
Contents: 10 μg / 100 μg (lyophilized)
Resuspend in 10 μl / 100 μl aqua bidest
Known applications: Dot blot1, immunohistochemistry (paraffin sections, 1:80-1:8000; electron microscopy, 1:600)1 ,2, 3, 4
This antibody has not been tested for use in all applications. This does not necessarily exclude its use in non-tested procedures. The stated dilutions are recommendations only. End users should determine optimal dilutions in their system using appropriate negative/positive controls.
Store at: 2-8 °C (lyophilized); - 20 °C (dissolved)
Repeated thawing and freezing must be avoided
References:
1. Eissele R, Goke R, Willemer s, Harthus HP, Vermeer H, Arnold R, Goke B (1992). Glucagon-like peptide-1 cells in the gastrointestinal tract and pancreas of rat, pig and man. Eur J Clin Invest 22(4): 283-291.
2. Theodorakis MJ, Carlson O, Michopoulos S, Doyle MiE, Juhaszova M, Petraki K, Egan JM (2006). Human duodenal enteroendocrine cells: source of both incretin peptides, GLP-1 and GIP. American Journal of Physiology-Endocrinology And Metabolism 290(3); E550-E559.
3. Nishimura K, Hiramatsu K, Monir MM, Takemoto C & Watanabe T (2013). Ultrastructural Study on Colocalization of Glucagon-Like Peptide (GLP)-1 with GLP-2 in Chicken Intestinal L-Cells. J. Vet. Med. Sci. 75: 1335-1339.
4. Watanabe T, Nishimura K, Monir MM, TakemotoC & Hiramatsu K (2014). Immunoelectron Microscopic Observation of Chicken Glucagon-ike Peptide (GLP)-1-Containing Cells in Tissues Derived from Thin Section, Paraffin Block and Conventional Method. J. Vet. Med. Sci. 76: 389-394.
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