Gene Information: Gene Name: EGFR, ERBB1
GenelD: 1956
Host: Mouse Balb/c
Clone no: EGF-R2
Isotype: IgG1
Matrix: Cell culture supernatant, Protein G purified, PBS pH 7.4, 0.1% NaN3
Specificity: Human EGF-Receptor (extracellular non ligand binding site)
There was no cross reactivity obtained with v-erb-B.
Contents: 10 μg / 100 μg (lyophilized)
Resuspend in 10 μl / 100 μl aqua bidest.
Known applications: ELISA1, 2, 3, immunohistochemistry (paraffin sections, cryosections), immunocytochemistry, flow cytometry
Antigen retrieval: Heat the deparaffinized, rehydrated sections 2x in fresh citrate buffer pH 6.0 for 5 min. in microwave
This antibody has not been tested for use in all applications. This does not necessarily exclude its use for non-tested procedures. The stated dilutions are recommendations only. We suggest that the applicant titrates the antibody in his/her system using appropriate negative/positive controls.
Store at: 2-8 °C (lyophilized); - 20 °C (dissolved)
Repeated thawing and freezing must be avoided
References:
1. Manneck H, Steinhilber G, Pollow K, Roos W, Ruppert J, Armbruster F (1993) Enzyme-linked
Immunosorbent Assay for the Determination of Epidermal Growth Factor Receptor. Klin Lab 39: 177-
182.
2. Reins HA, Steinhilber G, Freiberg B, Anderer FA (1993) Anti-epidermal growth factor receptor
monoclonal antibodies affecting signal transduction. J Cell Biochem 51(2): 236 -248.
3. Roth H-J, Seikel I, Schmidt B, Kloppinger M, Kasper U, Armbruster FP (1994). Is it necessary for a
quantitative EGF-receptor assay to detect the ligand bindin site? Comparison of methods: Radioligand
Binding Assay (RLBA) vs. IEMA Ligang Binding Site vs. IEMA Non-Ligand Binding Site. Klin Lab 40: 1025-1032
For research use only
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