ANTIBODY, MONOCLONAL
Catalog no.: AK3007.2
Immunogen: Human EGF-Receptor
Synonyms: Receptor tyrosine-protein kinase erbB-1, Proto-oncogene c-ErbB-1
Swiss-Prot No: P00533
Gene Information: Gene Name: EGFR, ERBB1
GenelD: 1956
Host: Mouse Balb/c
Clone no.: EGF-R1
Isotype: IgG1
Matrix: Cell culture supernatant, Protein G purified, PBS pH 7.4, 0.1% NaN3
Specificity: Human EGF-Receptor (extracellular ligand binding site)
There was no cross reactivity obtained with v-erb-B.
Contents: 100 μg (lyophilized)
Resuspend in 100 μl aqua bidest.
Known applications: ELISA1,2, immunohistochemistry (paraffin sections, 1.7 ng/ml PBS, 1% BSA), flow cytometry
This antibody has not been tested for use in all applications. This does not necessarily exclude its use for non-tested procedures. The stated dilutions are recommendations only. We suggest that the applicant titrates the antibody in his/her system using appropriate negative/positive controls.
Store at: 2-8 °C (lyophilized); - 20 °C (dissolved)
Repeated thawing and freezing must be avoided
References:
1. Manneck H, Steinhilber G, Pollow K, Roos W, Ruppert J, Armbruster F (1993) Enzyme-linked Immunosorbent Assay for the Determination of Epidermal Growth Factor Receptor. Klin Lab 39: 177-182.
2. Reins HA, Steinhilber G, Freiberg B, Anderer FA (1993) Anti-epidermal growth factor receptor monoclonal antibodies affecting signal transduction.J Cell Blochem 51(2): 236-248.
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