ANTIBODY, MONOCLONAL
Catalog no.: AA1004.1
Immunogen: 3-(p-hydroxyphenyl) propionic acid dimer KLH-conjugated
Host: Mouse
Clone no.: 1C3
Isotype: IgG2a(kappa)
Matrix: Protein A purified, 10mM PBS (pH 7.4)
Specificity: Specific for free dityrosine, 3-(p-hydroxyphenyl) propionic acid dimer, dityrosine-BSA conjugate, and dityrosine in proteins or peptides.
There was no cross reactivity detected for the following compounds: tyrosine, 3-nitrotyrosine, 3-chlorotyrosine, aminotyrosine, phosphotyrosine, 3-(p-hydroxyphenyl) propionic acid, phenylalanine, 3,4-dihydroxyphenylacetic acid (DOPA)
Contents: 20 μg (lyophilized)
Resuspend in 200 μl aqua bidest. for 100 μg/ml
Known applications: ELISA1, 2, Western Blot (4 μg/ml)3, 5, immunohistochemistry (paraffin sections, 1μg/ml)4, 6
This antibody has not been tested for use in all applications. This does not necessarily exclude its use in non-tested procedures. The stated dilutions are recommendations only. End users should determine optimal dilutions in their system using appropriate negative/positive controls.
Store at: - 20 °C
Repeated thawing and freezing must be avoided.
References:
1. Kato Y, Wu X, Naito M, Nomura H, Kitamoto N, Osawa T (2000). Immunochemical detection of protein dityrosine in atherosclerotic lesion of apo-E-deficient mice using a novel monoclonal antibody. Biochem Biophys Res Commun. 275 (1), p11-15.
2. Kato Y, Kitamoto N, Kawai Y, Osawa T (2001). The hydrogen peroxide/copper ion system, but not other metal-catalyzed oxidation systems, produces protein-bound dityrosine. Free Radical Biology and Medicine 31(5): 624-632.
3. Atwood CS, Perry G, Zeng H, Kato Y, Jones WD, Ling KQ, Huang X, Moir RD, Wang D, Sayre LM, Smith MA, Chen SG, Bush AI (2004). Copper mediates dityrosine cross-linking of Alzheimer's amyloid-beta. Biochemistry 43(2): 560-568.
4. Shiba Y, Kinoshita T, Chuman H, Taketani Y, Takeda E, Kato Y, Naito M, Kawabata K, Ishisaka A, Terao J, Kawai Y (2008). Flavonoids as Substrates and Inhibitors of Myeloperoxidase: Molecular Actions of Aglycone and Metabolites. Chemical Research in Toxicology 21(8): 1600-1609.
5. Suderman RJ, Dittmer NT, Kramer KJ, Kanost MR (2010). Model reactions for insect cuticle sclerotization: participation of amino groups in the cross-linking of Manduca sexta cuticle protein MsCP36. Insect Biochem Mol Biol 40(3): 252-258.
6. Kimoto Y, Sugiyama A, Nishinohara M, Asano A, Masuda A, Ochi T, Takeuchi T (2011). Expressions of protein oxidation markers, dityrosine and advanced oxidation protein products in Cisplatin-induced nephrotoxicity in rats. J Vet Med Sci 73(3): 403-407.
For research use only
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