yl23411永利(中国)有限公司-搜狗百科

Specificity: Specific for dihalogenated tyrosine. Recognizes dibromo-tyrosine (DiBrY), Nacetylated dichloro-tyrosine (NAcDiClY), diiodo-tyrosine (DiIY), Nbromo-succinimidetreated BSA, HOCltreated BSA and dibromotyrosine conjugated BSA. Bromotyrosine (BrY) is weakly recognized.

There was no cross reactivity obtained with dityrosineconjugated BSA, peroxynitritetreated BSA, tyrosine (Y), chlorotyrosine(ClY), 33’dityrosine (DiY), 3aminotyrosine (NH2Y), 3nitrotyrosine (NO2Y), phenylalanine (Phe), ophosphotyrosine, 3, 4dihydroxyphenylacetic acid (DOPA) and 2hydroxy4phenylacetic acid. No cross reactivity was obtained with brominated nucleic acids 5 bromouracil, 5-bromouridine, and 8-bromoguanosine.

Contents: 20 μg (lyophilized)

Resuspend in 200 μl aqua bidest. for 100 μg/ml of IgG

Known applications: ELISA1, Western Blot, immunohistochemistry (paraffin sections, 10 μg/ml)1

This antibody has not been tested for use in all applications. This does not necessarily exclude its use in non-tested procedures. The stated dilutions are recommendations only. End users should determine optimal dilutions in their system using appropriate negative/positive controls.

Store at: - 20 °C

Repeated thawing and freezing must be avoided

References:
1. Kato Y, Kawai Y, Morinaga H, Kondo H, Dozaki N, Kitamoto N, Osawa T. (2005). Immunogenicity of a brominated protein and successive establishment of a monoclonal antibody to dihalogenated tyrosine. Free Radic Biol Med 38(1): 24-31.

2. Wu W, Chen Y, d'Avignon A, Hazen SL. (1999). 3-Bromotyrosine and 3,5-dibromotyrosine are major products of protein oxidation by eosinophil peroxidase: potential markers for eosinophildependent tissue injury in vivo. Biochemistry 38(12):3538-48.

For research use only