ANTIBODY, POLYCLONAL
Catalog no.: AL1006.3 / AL1006.4
Immunogen Recombinant murine type VII collagen (aa 757-967) GST fusion protein
Synonyms: Long-chain collagen (LC collagen)
Swiss-Prot No: Q63870
Gene Information: Gene name: Col7a1
GeneID: 12836
Host: Rabbit
Matrix: IgG, Protein G purified from serum; PBS pH 7.2
Specificity: Recombinant murine type VII collagen (aa 757-967); murine 290 kDa type VII collagen (epidermal basement membrane / anchoring fibril component)
Cross-reacts with human skin.
Contents: 10 μg / 100 μg (lyophilized)
Resuspend in 10 μl / 100 μl aqua bidest.
Known applications: ELISA, immunohistochemistry (cryosections), induction of experimental EBA in mice1, 2
This antibody has not been tested for use in all applications. This does not necessarily exclude its use in non-tested procedures. The stated dilutions are recommendations only. End users should determine optimal dilutions/concentrations in their system using appropriate negative/positive controls.
Store at: 2-8 °C (lyophilized); - 20 °C (dissolved)
Repeated thawing and freezing must be avoided
References:
1. Sitaru C, Mihai S, Otto C, Chiriac MT, Hausser I, Dotterweich B, Saito H, Rose C, Ishiko A, Zillikens D (2005). Induction of dermal-epidermal separation in mice by passive transfer of antibodies specific to type VII collagen. J Clin Invest 115(4): 870-878.
2. Sesarman A, Sitaru AG, Olaru F, Zillikens D, Sitaru C (2008). Neonatal Fc receptor deficiency protects from tissue injury in experimental epidermolysis bullosa acquisita. J Mol Med 86(8): 951-959.
For research use only
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