Gene Information:
Gene Name:Col7a1
GenelD: 12836
Host: Rabbit
Matrix: lgG, Protein G purified from serum; PBS
Specificity: Recombinant murine type VII collagen (aa 757-967)
Cross reacts with murine and human type VII collagen. Not tested for reactivity with type VII collagen from other species.
Contents: 10 μg / 100 μg (lyophilized)
Resuspend in 10 μl / 100 μl aqua bidest.
Known applications: ELISA (1:5000), Western Blot (1:500)1, immunofluorescence (cryosections, mouse skin 1:1000, human skin 1:500)1
This antibody has not been tested for use in all applications. This does not necessarily exclude its use in non-tested procedures. The stated dilutions are recommendations only. End users should determine optimal dilutions in their system using appropriate negative/positive controls.
Store at: 2-8 °C (lyophilized); - 20 °C (dissolved)
Repeated thawing and freezing must be avoided
References:
1. Sitaru C, Mihai S, Otto C, Chiriac MT, Hausser I, Dotterweich B, Saito H, Rose C, Ishiko A, Zllikens D (2005). Induction of dermalepidermal separation in mice by passive transfer of antibodies specific to type VII collagen.J Clin Invest 115(4): 870-878.
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