This antibody has not been tested for use in all applications. This does not necessarily exclude its use in non-tested procedures. The stated dilutions are recommendations only. End users should determine optimal dilutions in their system using appropriate negative/positive controls.
Store at: 2-8 °C (lyophilized) -20 °C (dissoveld)
Repeated thawing and freezing must be avoided
References:
1. Hirose M, Recke A, Beckmann T, Shimizu A, Ishiko A, Bieber K, Westermann Jr, Zillikens D, Schmidt E, Ludwig RJ (2011). Repetitive Immunization Breaks Tolerance to Type XVII Collagen and Leads to Bullous Pemphigoid in Mice. The Journal of Immunology 187(3): 1176-1183.
2. Hellberg L, Samavedam UK, Holdorf K, Hansel M, Recke A, Beckmann T, Steinhorst K, Boehncke WH, Kirchner T, Mockel N, Solbach W, Zillikens D, Schmidt E, Ludwig RJ, Laskay T (2013). Methylprednisolone blocks autoantibody-induced tissue damage in experimental models of bullous pemphigoid and epidermolysis bullosa acquisita through inhibition of neutrophil activation. J Invest Dermatol 133(10): 2390-2399.
3. Schulze FS, Beckmann T, Nimmerjahn F, Ishiko A, Collin M, Kohl J, Goletz S, Zillikens D, Ludwig R & Schmidt E (2014) Fcγ Receptors II and IV Mediate Tissue Destruction in a Novel Adult Mouse Model of Bullous Pemphigoid. Am. J. Pathol. 184: 2185-2196.
4. Akbarzadeh R, Yu X, Vogl T, Ludwig RJ, Schmidt E, Zilikens D & Petersen F (2015) Myeloid-related proteins-8 and -14 are expressed but dispensable in the pathogenesis of experimental epidermolysis bullosa acquisita and bullous pemphigoid. J. Dermatol. Sci: 8-15 [epub ahead of prinf].
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