Swiss-Prot No: Q9UMD9
Gene Information:
Gene Name: COL17A1, BP180, BPAG2
GenelD: 1308
Host: Rabbit
Matrix: lgG, Protein G purified from serum; 50 mM Tris pH 7.4.
Specificity: Human type XVII collagen
Contents: 10 μg / 100 μg (lyophilized)
Resuspend in 10 μl / 100 μl aqua bidest.
Known applications: ELISA (1:5000), Western Blot (1:500)2, immunohistochemistry (cryosections, 1:100), immunocytochemistry3
This antibody has not been tested for use in all applications. This does not necessarily exclude its use in non-tested procedures. The stated dilutions are recommendations only. End users should determine optimal dilutions/concentrations in their system using appropriate negative/ positive controls.
Store at: 2-8 °C (lyophilized); - 20 °C (dissolved)
Repeated thawing and freezing must be avoided
References:
1. Sitaru C, Schmidt E, Petermann S, Munteanu LS, Brocker EB, Zllikens D (2002). Autoantibodies to bullous pemphigoid antigen 180 induce dermalepidermal separation in cryosections of human skin. J Invest Dermatol. 118:664-71.
2. Hirako Y, Yoshino K, Zllikens D, Owaribe K (2003). Extracellular cleavage of bullous pemphigoid antigen 180/type XVII collagen and its involvement in hemidesmosomal disassembly. J Biochem 133(2): 197-206.
3. Hirako Y, Nishizawa Y, Sitaru C, Opitz A, Marcus K, Meyer HE, Butt E, Owaribe K, Zllikens D (2003). The 97-kDa (LABD97) and 120-kDa (LAD-1) fragments of bullous pemphigoid antigen 180/type XVII collagen have different N-termini. J Invest Dermatol. 121:1554-6.
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