ANTIBODY, POLYCLONAL
Catalog no: AST1017.1 / AST1017.2
Immunogen: Synthetic human CgB (aa 312-331) KLH-conjugated (YRASEEPEYGEEIKGYPGV)
Synonyms: Secretogranin-1, Chromogranin-B, CgB, Secretogranin I, Sgl
Swiss- Prot No: P05060
Gene Information:
Gene Name: CHGB
GenelD: 1114
Host: Rabbit
Matrix: Serum
Specificity: Human CgB (aa 312-331)
There was no cross reactivity detected with human CgB peptides (aa 1-16; 16-37; 153-167; 244-255; 259-270; 406-417; 420-432; 480- 493; 506-517; 542-552; 555-565; 568-577; 580-593; 622-632; 647-657).
Contents: 20 μl / 100 μl (lyophilized)
Resuspend in 20 μl / 100 μl aqua bidest.
Known applications: RIA2, 4, Western Blot, immunohistochemistry (paraffin sections, 1:40-400)1, 3
This antibody has not been tested for use in all applications. This does not necessarily exclude its use in non-tested procedures. The stated dilutions are recommendations only. End users should determine optimal dilutions in their system using appropriate negative/positive controls.
Store at: 2-8 °C (lyophilized); - 20 °C (dissolved)
Repeated thawing and freezing must be avoided
References:
1. Stridsberg M, Oberg K, Li Q, Engstrom U & Lundqvist G (1995) Measurements of chromogranin A, chromogranin B (secretogranin I), chromogranin C (secretogranin I) and pancreastatin in plasma and urine from patients with carcinoid tumours and endocrine pancreatic tumours. J. Endocrinol. 144: 49-59.
2. Stridsberg M, Angeletti RH & Helle KB (2000) Characterisation of N-terminal chromogranin A and chromogranin B in mammals by region-specific radioimmunoassays and chromatographic separation methods. J. Endocrinol. 165: 703-714.
3. PortelaGomes GM & Stridsberg M (2002) Region-specific antibodies to chromogranin B display various immunostaining patterns in human endocrine pancreas. J. Histochem. Cytochem. 50: 1023-1030.
4. Stridsberg M, Eriksson B, Oberg K & Janson ET (2005) A panel of 13 region-specific radioimmunoassays for measurements of human chromogranin B. Regul. Pept. 125: 193-199.
For research use only
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