ANTIBODY, POLYCLONAL
Catalog no.: AST1016.1 / AST1016.2
Immunogen: Synthetic human CgB (aa 259-270) KLH-conjugated (TRPRHHHGRSRPYC)
Underlined amino acids were substituted or added for technical reasons.
Synonyms: Secretogranin-1, Chromogranin-B, CgB, Secretogranin , Sgl
Swiss-Prot No: P05060
Gene Information:
Gene Name: CHGB
GenelD: 1114
Host: Rabbit
Matrix: Serum
Specificity: Human CgB (aa 259-270)
There was no cross reactivity detected with human CgB peptides (aa 1-16; 16-37; 153-167; 244-255; 312-331; 406-417; 420-432; 480-493; 506-517; 542-552; 555-565; 568-577; 580-593; 622-632; 647-657).
Contents: 20 μl / 100 μl (lyophilized)
Resuspend in 20 μl / 100 μl aqua bidest.
Known applications: Western Blot, immunohistochemistry (paraffin sections, 1:20-200)1
For staining of paraffin sections microwave antigen retrieval in citrate buffer pH 6.0 is recommended
This antibody has not been tested for use in all applcations. This does not necessarily exclude its use in non-tested procedures. The stated dilutions are recommendations only. End users should determine optimal dilutions in their system using appropriate negative/positive controls.
Store at: 2-8 °C (lyophilized); - 20 °C (dissolved)
Repeated thawing and freezing must be avoided
References:
1. Portela-Gomes GM & Stridsberg M (2002) Region-specific antibodies to chromogranin B display
various immunostaining patterns in human endocrine pancreas. J. Histochem. Cytochem. 50: 1023-
1030.
2. Stridsberg M, Eriksson B, Oberg K& Janson ET (2005) A panel of 13 region-specific
radioimmunoassays for measurements of human chromogranin B. Regul. Pept. 125: 193-199.
For research use only
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