ANTIBODY, POLYCLONAL
Catalog no.: AST1011.1 / AST1011.2
Immunogen: Synthetic human CgA (aa 411-424) KLH-conjugated (PEDQELESLSAIEAYC)
Underlined amino acids were substituted or added for technical reasons.
Synonyms: Pituitary secretory protein I, SP-I
Swiss-Prot No: P10645
Gene Information: Gene Name: CHGA
GeneID: 1113
Host: Rabbit
Matrix: Serum
Specificity: Human CgA (aa 411-424)
There was no cross reactivity detected with human CgA peptides (aa 1-17; 17-38; 63-76;100-113; 116-130; 176-195; 238-247; 284-301; 324-337; 361-372; 375-384).
Contents: 20 μl / 100 μl (lyophilized)
Resuspend in 20 μl / 100 μl aqua bidest.
Known applications: RIA5, 6, Western Blot, immunohistochemistry (paraffin sections, 1:80-1000)1, 2, 3, 4, 6
This antibody has not been tested for use in all applications. This does not necessarily exclude its use in non-tested procedures. The stated dilutions are recommendations only. End users should determine optimal dilutions in their system using appropriate negative/positive controls.
Store at: 2-8 °C (lyophilized); - 20 °C (dissolved)
Repeated thawing and freezing must be avoided
References:
1. Portela-Gomes GM & Stridsberg M (2001) Selective processing of chromogranin A in the different islet cells in human pancreas. J. Histochem. Cytochem. 49: 483–490.
2. Portela-Gomes GM, Grimelius L, Johansson H, Wilander E & Stridsberg M (2001) Chromogranin A in human neuroendocrine tumors: an immunohistochemical study with region-specific antibodies. Am. J. Surg. Pathol. 25: 1261–1267
3. Portela-Gomes GM & Stridsberg M (2002) Chromogranin A in the human gastrointestinal tract: an immunocytochemical study with region-specific antibodies. J. Histochem. Cytochem. 50: 1487–1492.
4. Jin L, Zhang S, Bayliss J, Scheithauer B, Qian X, Kobayashi I, Stridsberg M & Lloyd R V (2003) Chromogranin a processing in human pituitary adenomas and carcinomas: analysis with regionspecific antibodies. Endocr. Pathol. 14: 37–48.
5. Stridsberg M, Eriksson B, Öberg K & Janson ET (2004) A panel of 11 region-specific radioimmunoassays for measurements of human chromogranin A. Regul. Pept. 117: 219–227.
6. Tartaglia A, Portela-Gomes GM, Öberg K, Vezzadini P, Foschini MP & Stridsberg M (2006) Chromogranin A in gastric neuroendocrine tumours: An immunohistochemical and biochemical study with region-specific antibodies. Virchows Arch. 448: 399–406.
For research use only
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