ANTIBODY, POLYCLONAL
Catalog no.: AST1009.1 / AST1009.2
Immunogen: Synthetic human CgA (aa 361-372) KLH-conjugated (CRAYGFRGPGPQL)
Underlined amino acids were substituted or added for technical reasons.
Synonyms: Pituitary secretory protein I, SP-I
Swiss-Prot No: P10645
Gene Information: Gene Name: CHGA
GeneID: 1113
Host: Rabbit
Matrix: Serum
Specificity: Human CgA (aa 361-372)
There was no cross reactivity detected with human CgA peptides (aa 1-17; 17-38; 63-76; 100-113; 116-130; 176-195; 238-247; 284-301; 324-337; 361-372; 375-384; 411-424), intact human CgB, insulin, glucagon, somatostatin(aa 1-14), or pancreatic polypeptide.
Contents: 20 μl / 100 μl (lyophilized)
Resuspend in 20 μl / 100 μl aqua bidest.
Known applications: RIA4, 5, Western Blot, immunohistochemistry (paraffin sections, 1:80-1000)1, 2, 3, 4, 5
For staining of paraffin sections microwave antigen retrieval in citrate buffer pH 6.0 is recommended1
This antibody has not been tested for use in all applications. This does not necessarily exclude its use in non-tested procedures. The stated dilutions are recommendations only. End users should determine optimal dilutions in their system using appropriate negative/positive controls.
Store at: 2-8 °C (lyophilized); -20 °C (dissolved)
Repeated thawing and freezing must be avoided
References:
1. Portela-Gomes GM & Stridsberg M (2001) Selective processing of chromogranin A in the different islet cells in human pancreas. J. Histochem. Cytochem. 49: 483–490.
2. Portela-Gomes GM & Stridsberg M (2002) Chromogranin A in the human gastrointestinal tract: an immunocytochemical study with region-specific antibodies. J. Histochem. Cytochem. 50: 1487–1492.
3. Jin L, Zhang S, Bayliss J, Scheithauer B, Qian X, Kobayashi I, Stridsberg M & Lloyd R V (2003) Chromogranin a processing in human pituitary adenomas and carcinomas: analysis with regionspecific antibodies. Endocr. Pathol. 14: 37–48.
4. Stridsberg M, Eriksson B, Öberg K & Janson ET (2004) A panel of 11 region-specific radioimmunoassays for measurements of human chromogranin A. Regul. Pept. 117: 219–227.
5. Tartaglia A, Portela-Gomes GM, Öberg K, Vezzadini P, Foschini MP & Stridsberg M (2006) Chromogranin A in gastric neuroendocrine tumours: An immunohistochemical and biochemical study with region-specific antibodies. Virchows Arch. 448: 399–406.
For research use only
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