ANTIBODY, POLYCLONAL
Catalog no.: AST1000.1 / AST1000.2
Immunogen: Synthetic human CgA (aa 1-17) KLH-conjugated (LPVNSPMNKGDTEVMYC)
Underlined amino acids were substituted or added for technical reasons.
Synonyms: Pituitary secretory protein I, SP-I
Swiss-Prot No: P10645
Gene Information:
Gene Name: CHGA
GenelD: 1113
Host: Rabbit
Matrix: Serum
Specificity: Human Chromogranin A (aa 1-17); bovine, porcine, equine, and sheep CgA (aa 1-17)
There was no cross reactivity detected with human CgA peptides (aa 17-38; 63-76; 100-113; 116-130; 176-195; 238-247; 284-301; 324-337; 361-372;375-384; 411-424), intact human or bovine CgA, human CgB peptide (aa 16-37), intact human CgB, insulin, glucagon, somatostatin (aa 1-14), or pancreatic polypeptide.
Contents: 20 μl / 100 μl (lyophilized)
Resuspend in 20 μl / 100 μl aqua bidest.
Known applications: RIA1, 5, 6, Western Blot, immunohistochemistry (paraffin sections, 1:80-1000)2, 3, 4, 6
This antibody has not been tested for use in all applications. This does not necessarily exclude its use in non-tested procedures. The stated dilutions are recommendations only. End users should determine optimal dilutions in their system using appropriate negative/positive controls.
Store at: 2-8 °C (lyophilized);- 20 °C (dissolved)
Repeated thawing and freezing must be avoided
References:
1. Stridsberg M, Angeletti RH & Helle KB (2000) Characterisation of N-terminal chromogranin A and chromogranin B in mammals by region-specific radioimmunoassays and chromatographic separation methods. J Endocrinol. 165: 703-714.
2. Portela-Gomes GM & Stridsberg M (2001) Selective processing of chromogranin A in the dfferent islet cells in human pancreas. J. Histochem. Cytochem.49: 483-490.
3. Portela-Gomes GM & Stridsberg M (2002) Chromogranin A in the human gastrointestinal tract: an immunocytochemical study with region-specific antibodies. J. Histochem. Cytochem. 50: 1487-1492.
4. Jin L, Zhang S, Bayliss J, Scheithauer B, Qian X, Kobayashi I, Stridsberg M & Lloyd R V (2003) Chromogranin a processing in human pituitary adenomas and carcinomas: analysis with region-specific antibodies. Endocr. Pathol. 14: 37-48.
5. Stridsberg M, Eriksson B, Oberg K & Janson ET (2004) A panel of 11 region-specific radioimmunoassays for measurements of human chromogranin A. Regul. Pept. 117: 219-227.
6. Tartaglia A, Portela-Gomes GM, Oberg K, Vezzadini P, Foschini MP & Stridsberg M (2006) Chromogranin A in gastric neuroendocrine tumours: An immunohistochemical and biochemical study with region-specific antibodies. Virchows Arch. 448: 399-406.
For research use only
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