Clone no.: FW6
Isotype: lgM
Matrix: Cell culture supernatant, purified, PBS pH 7.4
Specificity: Alpha-3, 4-monofucosylated Polylactosaminoglycans on mucins, oncofetal antigen
Contents: 10 μg / 100 μg (lyophilized)
Resuspend in 10 μl / 100 μl aqua bidest.
Known applications: ELISA, Western Blot2, immunohistochemistry (paraffin sections)1,2,3, immunocytochemistry1
This antibody has not been tested for use in all applications. This does not necessarily exclude its use in non-tested procedures. The stated dilutions are recommendations only. End users should determine
optimal dilutions in their system using appropriate negative/positive controls.
Store at: 2-8 °C (lyophilized); - 20 °C (dissolved)
Repeated thawing and freezing must be avoided
References:
1. Schwonzen M, Schmits R, Baldus SE, Vierbuchen M, Hanisch FG, Pfreundschuh M, Diehl V, Bara J, Uhlenbruck G (1992). Monoclonal antibody FW6 generated against a mucin-carbohydrate of human amniotic fluid recognises a colonic tumour-associated epitope. Br J Cancer 65(4): 559-565.
2. Hanisch FG, Heimbichel G, Baldus SE, Uhlenbruck G, Schmits R, Pfreundschuh M, Schwonzen M, Vierbuchen M, Bara J, Peter-Katalinic J (1993). Monoclonal antibody FW6 defines an epitope on alpha 3/4-monofucosylated polylactosaminoglycans expressed by fetal and colon carcinoma associated
mucins. Cancer Res 53(18): 4367-4375.
3. Baldus SE, Vierbuchen M, Hanisch FG, Schwonzen M, Fischer R (1995). Expression of alpha 3/4-monofucosylated polylactosaminoglycan epitope, as defined by monoclonal antibody FW6, is a marker of the colorectal adenoma- carcinoma sequence. Cancer 76(6): 954-960.
For research use only
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