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Pretreatment of slides by pressure cooking (5 min at 125°C in TRIS/EDTA pH 8.5) is highly recommended.
This antibody has not been tested for use in all applications. This does not necessarily exclude its use in non-tested procedures. The stated dilutions are recommendations only. End users should determine optimal dilutions in their system using appropriate negative/positive controls.

Store at: 2-8 °C (do not freeze)

References:

1. Regele H, Exner M, Watschinger B, Wenter C, Wahrmann M, Osterreicher C, Saemann MD, Mersich N, Horl WH, Zlabinger GJ, Bohmig GA (2001). Endothelial C4d deposition is associated with inferior kidney allograft outcome independently of cellular rejection. Nephrol Dial Transplant 16(10); 2058-2066.

2. Bohmig GA, Regele H, Exner M, Derhartunian V, Kletzmayr J, Saemann MD, Horl WH, Druml W, Watschinger B (2001). C4d-positive acute humoral renal allograft rejection: effective treatment by immunoadsorption. J Am Soc Nephrol 12(11): 2482- 2489.

3. Regele H, Bohmig GA, Habicht A, Gollowitzer D, Schillinger M, Rockenschaub S, Watschinger B, Kerjaschki D, Exner M (2002). Capillary deposition of complement split product C4d in renal allografts is associated with basement membrane injury in peritubular and glomerular capillaries: a contribution of humoral immunity to chronic allograft rejection. J Am Soc Nephrol 13(9): 2371-2380.

4. Bohmig GA, Exner M, Habicht A, Schillinger M, Lang U, Kletzmayr J, Saemann MD, Horl WH, Watschinger B, Regele H (2002). Capillary C4d deposition in kidney algrafts: a specific marker of alloantibody-dependent graft injury. J Am Soc Nephrol 13(4): 1091-1099.

For research use only