ANTIBODY, MONOCLONAL
Catalog no.: AA1005.1 / AA1005.2
Immunogen: 8-OHdG keyhole limpet hemocyanin-conjugated
Host: Mouse
Clone no.: N45.1
Isotype: IgG1(kappa)
Matrix: Ascites, ammonium sulphate purified, 10 mM Phosphate buffered saline, pH7.4, 1.0% BSA
Specificity: 8-OHdG in human, rabbit, rat and mouse tissue and cells
There was no cross reactivity obtained with 19 analogues of 8-OHdG: guanosine (G), 7-methyl-G, 6-SH-G, 8-bromo-G, dA, dC, dT, dI, dU, dG, O6-methyl-dG, 8-OHdA, guanine (Gua), O6-methyl-Gua, 8-OHGua, uric acid, urea, creatine, creatinine. Only 8-sulfhydryl-G and 8-OHG demonstrate minimal cross-reactivity (less than 1%).
Contents: 20 μg / 100 μg (lyophilized)
Resuspend in 200 μl / 1000 μl aqua bidest. for 100 μg/ml
Known applications: ELISA3, immunohistochemistry (paraffin sections, 3-5 μg/ml)1, 2, 3, 4, 5, 6, immunocytochemistry7
This antibody has not been tested for use in all applications. This does not necessarily exclude its use in non-tested procedures. The stated dilutions are recommendations only. End users should determine optimal dilutions in their system using appropriate negative/positive controls.
Store at: - 20 °C
Repeated thawing and freezing must be avoided
References:
1. Hattori Y, Nishigori C, Tanaka T, Uchida K, Nikaido O, Osawa T, Hiai H, Imamura S, Toyokuni S (1996). 8-hydroxy-2'-deoxyguanosine is increased in epidermal cells of hairless mice after chronic ultraviolet B exposure. J Invest Dermatol 107(5): 733-737.
2. Tanaka T, Nishiyama Y, Okada K, Hirota K, Matsui M, Yodoi J, Hiai H, Toyokuni S (1997). Induction and nuclear translocation of thioredoxin by oxidative damage in the mouse kidney: independence of tubular necrosis and sulfhydryl depletion. Lab Invest 77(2): 145-155.
3. Toyokuni S, Tanaka T, Hattori Y, Nishiyama Y, Yoshida A, Uchida K, Hiai H, Ochi H, Osawa T (1997). Quantitative immunohistochemical determination of 8-hydroxy-2'-deoxyguanosine by a monoclonal antibody N45.1: its application to ferric nitrilotriacetate-induced renal carcinogenesis model. Lab Invest 76(3): 365-74.
4. Ihara Y, Toyokuni S, Uchida K, Odaka H, Tanaka T, Ikeda H, Hiai H, Seino Y, Yamada Y (1999). Hyperglycemia causes oxidative stress in pancreatic beta-cells of GK rats, a model of type 2 diabetes. Diabetes 48(4): 927-932.
5. Kondo S, Toyokuni S, Iwasa Y, Tanaka T, Onodera H, Hiai H, Imamura M (1999). Persistent oxidative stress in human colorectal carcinoma, but not in adenoma. Free Radic Biol Med 27(3-4): 401-410.
6. Ichiseki T, Kaneuji A, Katsuda S, Ueda Y, Sugimori T, Matsumoto T (2005). DNA oxidation injury in bone early after steroid administration is involved in the pathogenesis of steroid-induced osteonecrosis. Rheumatology (Oxford) 44(4): 456-460.
7. Lee YA, Cho EJ, Yokozawa T (2008). Protective effect of persimmon (Diospyros kaki) peel proanthocyanidin against oxidative damage under H2O2-induced cellular senescence. Biol Pharm Bull 31(6): 1265-1269.
For research use only
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