ANTIBODY, MONOCLONAL
Catalog no.: AA1006.1 / AA1006.2
Immunogen: 4-HNE-modified Keyhole Limpet Hemocyanin
Host: Mouse
Clone no.: HNEJ-2
Isotype: IgG1(kappa)
Matrix: Ascites, ammonium sulfate purified, 50 mM Tris-buffered saline (TBS)
Specificity: 4-hydroxy-2-nonenal (4-HNE)
This antibody shows almost negligible reactivity with proteins that were treated with other aldehydes, such as 2-nonenal, 2-hexenal, 1-hexanal, 4-hydroxy-2-hexenal, formaldehyde, or glutaraldehyde
Contents: 20 μg / 100 μg (lyophilized)
Resuspend in 200 μl / 1000 μl aqua bidest. for 100 μg/ml
Known applications: ELISA1, Western Blot (15 μg/ml)1, immunohistochemistry (paraffin section, 10 μg/ml; cryosections)2, 3, 4
This antibody has not been tested for use in all applications. This does not necessarily exclude its use in non-tested procedures. The stated dilutions are recommendations only. End users should determine optimal dilutions in their system using appropriate negative/positive controls.
Store at: - 20 °C
Repeated thawing and freezing must be avoided
References:
1. Toyokuni S, Miyake N, Hiai H, Hagiwara M, Kawakishi S, Osawa T, Uchida K (1995). The monclonal antibody specific for the 4-hydroxy-2-nonenal histidine adduct. FEBS Lett. 359, p189-191.
2. Tanaka T, Nishiyama Y, Okada K, Hirota K, Matsui M, Yodoi J, Hiai H, Toyokuni S (1997). Induction and nuclear translocation of thioredoxin by oxidative damage in the mouse kidney: independence of tubular necrosis and sulfhydryl depletion. Lab. Invest. 77(2), p145-155.
3. Kondo S, Toyokuni S, Iwasa Y, Tanaka T, Onodera H, Hiai H, Imamura M (1999). Persistent oxidative stress in human colorectal carcinoma, but not in adenoma. Free Radic Biol Med 27(3-4): 401-410.
4. Yamada S, Funada T, Shibata N, Kobayashi M, Kawai Y, Tatsuda E, Furuhata A, Uchida K (2004). Protein-bound 4-hydroxy-2-hexenal as a marker of oxidized n-3 polyunsaturated fatty acids. J Lipid Res 45(4): 626-634.
For research use only
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