ANTIBODY, MONOCLONAL
Catalog no.: AA1010.1
Immunogen: HHE-modified KLH
Host: Mouse Balb/c
Clone no.: HHE53
Isotype: IgG1 kappa
Matrix: Ascites, Protein A purified, 10 mM PBS, 0.1% NaN3, 0.5% BSA
Specificity: HHE-modified protein, especially HHE-histidine Michael adduct

Contents: 30 μg (frozen; 100 μg/ml)
Known applications: ELISA (50 ng/ml)1, 2, Western Blot (1 μg/ml)1, immunohistochemistry
(paraffin sections, 0.5 - 1 μg/ml; cryosections)1, 2
This antibody has not been tested for use in all applications. This does not necessarily exclude
its use in non-tested procedures. The stated dilutions are recommendations only. End users
should determine optimal dilutions in their system using appropriate negative/positive controls.
Store at: -20 °C
Repeated thawing and freezing must be avoided
References:
1. Yamada S, Funada T, Shibata N, Kobayashi M, Kawai Y, Tatsuda E, Furuhata A, Uchida K (2004)
Protein-bound 4-hydroxy-2-hexenal as a marker of oxidized n-3 polyunsaturated fatty acids. J
Lipid Res 45(4): 626-634.
2. Shibata N, Yamada S, Uchida K, Hirano A, Sakoda S, Fujimura H, Sasaki S, Iwata M, Toi S,
Kawaguchi M, Yamamoto T, Kobayashi M (2004). Accumulation of protein-bound 4-hydroxy-2-
hexenal in spinal cords from patients with sporadic amyotrophic lateral sclerosis. Brain Res
1019(1-2): 170-177.
For research use only